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ifc controller hx  (fluidigm)


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Bioz Manufacturer Symbol fluidigm manufactures this product  
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    Structured Review

    fluidigm ifc controller hx
    Ifc Controller Hx, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 3825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ifc+controller+hx/Biomark/us12247982-452-10-13
    Average 96 stars, based on 3825 article reviews
    ifc controller hx - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Negative Control:

    Article Title: Correlating mechanical and gene expression data on the single cell level to investigate metastatic phenotypes
    Article Snippet: Amplified single cell samples were combined with SsoFast EvaGreen Supermix with Low ROX (BioRad) and DNA Binding Dye (Fluidigm) and each primer pair was diluted and combined with an assay-loading reagent (Fluidigm). .. The 96.96 Dynamic Array integrated fluidic chip (Fluidigm) was loaded with 96 samples (including a positive and negative control) and 96 assays with the IFC Controller HX (Fluidigm). .. Using a BioMark machine (Fluidigm), we performed 40 cycles of PCR and a melt curve analysis.

    Article Title: Agricultural pesticides pose a continuous ecotoxicological risk to aquatic organisms in a tropical horticulture catchment
    Article Snippet: Amplified single cell samples were combined with SsoFast EvaGreen Supermix with Low ROX (BioRad) and DNA Binding Dye (Fluidigm) and each primer pair was diluted and combined with an assay-loading reagent (Fluidigm). .. The 96.96 Dynamic Array integrated fluidic chip (Fluidigm) was loaded with 96 samples (including a positive and negative control) and 96 assays with the IFC Controller HX (Fluidigm). .. Using a BioMark machine (Fluidigm), we performed 40 cycles of PCR and a melt curve analysis.

    Real-time Polymerase Chain Reaction:

    Article Title: Markers selectively deregulated in tumor-infiltrating regulatory T cells
    Article Snippet: A 2.5 μl aliquot of each 20×TaqMan assay was mixed with 2.5 μl of Fluidigm's “assay loading agent” and individually inserted into one of the chip “assay” inlets. .. Samples and probes were loaded into 96×96 chips using an IFC Controller HX (Fluidigm), then transferred to a BioMark real-time PCR reader (Fluidigm) following manufacturer's instructions. ..

    other:

    Article Title: Methods and compositions to maintain stem cell quiescence
    Article Snippet: Pre-amplified cDNAs were treated with Exonuclease I (2.52 μl water, 0.36 μl buffer, 0.72 μl exonuclease I (New England BioLabs, PN M0293L)) for 30 minutes at 37° C., followed by 15 minutes inactivation at 80° C. Exonuclease treated cDNAs were diluted 5× in DNA suspension buffer (TEKnova, PN T0221) and stored at −80° C. To perform the Q-RT-PCR on the Fluidigm Biomark HD, sample mixes were prepared with 2.7 μl of diluted cDNAs mixed with 3.0 μl of 2× SsoFast EvaGreen Supermix with low ROX (Bio-Rad, PN 172-5211) and 0.3 μl of 20×DNA Binding Dye Sample loading reagent (Fluidigm, PN 100-0388), vortexed and spun down.



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